Sera, one of the three largest monasteries of Gelugpa, sits at the foothills of Tatipu. It is as prestigious as Drepung and Ganden, which both have longer histories. Sera, in Tibetan, means "Wild Rose Garden" since opulent wild rose woods once grew around it. A legend says that Tsong Khapa and his two disciples traveled in the area, spreading their religion. One day, they heard a horse whinnying underground when they were taking a walk in the rose woods. They dug up a statue of Hynagriva (a horse-headed demon-god) and Tsong Khapa began construction of a monastery to enshrine Hynagriva. However, the truth is that in 1414, Jamchen Chojey (or Sakya Yeshe), one of Tsong Khapa's disciples, visited Emperor Chengzu as Tsong Khapa's emissary. The Emperor Chengzu granted him a title of Dharma King of Great Mercy, sutras, and a set of sandalwood Arhats. In order to preserve them, Tsong Khapa instructed Jamchen Chojey to build a monastery to house the treasures. The Sera monastery was completed in 1419.

Sera is designed around a Main Assembly Hall, or Tshomchen in Tibetan, which is the grandest hall of Sera, occupying a floor space of 1,000 square meters. The four-storied hall has four chapels in which Arhats, Manjushri, Tsong Khapa, and Chenrezi are enshrined. Later, a huge Maitreya was enshrined in the hall during the reign of the Seventh Dalai Lama. The valuable Buddhist sutras that Jamchen Chojey brought back from Beijing are kept in a sutra pigeonhole adjacent to the hall.